Adli, Mazhar

Mazhar Adli

Mazhar Adli

Primary Appointment

Assistant Professor, Biochemistry and Molecular Genetics

Education

  • MSc, The Biological Sciences and Bioengineering Program, Sabanci University
  • PhD, Molecular Biology, University of North Carolina at Chapel Hill
  • Postdoc, Epogenomcis, Harvard Medical School, Mass general Hosptal, Broad Institute of Harvad and MIT

Contact Information

1340 Jefferson Park Ave, Jordan HAll
Charlottesville, VA 22908
Telephone: 4342438567
Email: adli@virginia.edu
Website: http://www.faculty.virginia.edu/adli/

Research Interests

Understanding and manipulating the genetics and epigenetics information to study stem cells, differentiation and cancer

Research Description

The human body contains more than 200 different cell types. Although these cells are genotypically the same, i.e., they posses the same genetic information, they are functionally and phenotypically different. Our lab integrates genomic and epigenomic technologies such as RNA-Seq and ChIP-Seq as well as imaging technologies (Flow cytometry, confocal microscopy) to understand how chromatin architecture is organized and functions differently in diverse cellular states. We are particularly focused on studying chromatin biology during normal and malignant development. Genetic information is encoded in four letters of A G C and T. All cells in the body 'read' this four letter genetic information to function. There are multiple levels of regulation during this process of 'reading' the genetic information. The primary level of regulation takes place at the DNA sequence level, in which the four letters of nucleic acids are organized in certain combinations and function as 'regulatory DNA elements' such as promoters, genes and enhancers. The secondary level of organization and regulation is controlled at the chromatin level where DNA is wrapped around special proteins called 'histone' proteins, forming nucleosomes. Nucleosomes can tightly or loosely package DNA and hence provide differential accessibility of genomic information. The tertiary level of genome regulation is dictated by the organization of nuclear architecture and long-range chromosomal interactions. These latter two modes of genome regulation are considered epigenetic mechanisms. Epigenetics (Epi-Â in Greek means above or over) can be defined in its simplest form as the study of heritable changes in gene expression and cellular states that are not caused by changes in genetic information. There are several sources of epigenetic information. Among those, methylation of DNA cytosine residues and post-translation modifications on histone proteins are relatively better-understood components of epigenetic information. Studying these epigenetic information at the whole genome level is called Epigenomics. The study of the epigenomic maps of diverse histone modifications and DNA methylation provide unprecedented information about global chromatin landscape, organization and function. Our lab is focused on the role chromatin organization in genome regulation during normal and malignant development such as cancer. We aim to identify epigenetic mechanisms and epigenomic features that will help us better understand the dynamic chromatin structure, function and nuclear organization during cellular differentiation programs such as stem cell self renewal and differentiation. In the lab, we apply state-of-the-art genomic and epigenomic tools and seek to develop additional technologies to answer the following specific questions.
  • How are chromatin and cellular states established during normal development?
  • What are the epigenetic determinants of differentiation programs of stem and progenitor cells?
  • How does aberrant regulation of epigenetic information lead to cellular transformation and malignant development?
  • How can we study chromatin structure in rarely available but biologically important cell populations and ultimately at single-cell-level?

Selected Publications